Localization of HLA-A2.1-restricted T cell epitopes in the circumsporozoite protein of Plasmodium falciparum.
Identifieur interne : 004031 ( Main/Exploration ); précédent : 004030; suivant : 004032Localization of HLA-A2.1-restricted T cell epitopes in the circumsporozoite protein of Plasmodium falciparum.
Auteurs : U. Blum-Tirouvanziam [Suisse] ; C. Servis ; A. Habluetzel ; D. Valmori ; Y. Men ; F. Esposito ; L. Del Nero ; N. Holmes ; N. Fasel ; G. CorradinSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1995.
Descripteurs français
- KwdFr :
- Activation des lymphocytes, Animaux, Antigène HLA-A2 (immunologie), Cartographie épitopique, Cytotoxicité immunologique, Données de séquences moléculaires, Humains, Immunité cellulaire, Interféron gamma (biosynthèse), Liaison aux protéines, Lymphocytes T (immunologie), Paludisme à Plasmodium falciparum (immunologie), Peptides (), Peptides (immunologie), Peptides (métabolisme), Plasmodium falciparum (immunologie), Protéines de protozoaire (immunologie), Souris, Souris transgéniques, Séquence d'acides aminés, Épitopes.
- MESH :
- biosynthèse : Interféron gamma.
- immunologie : Antigène HLA-A2, Lymphocytes T, Paludisme à Plasmodium falciparum, Peptides, Plasmodium falciparum, Protéines de protozoaire.
- métabolisme : Peptides.
- Activation des lymphocytes, Animaux, Cartographie épitopique, Cytotoxicité immunologique, Données de séquences moléculaires, Humains, Immunité cellulaire, Liaison aux protéines, Peptides, Souris, Souris transgéniques, Séquence d'acides aminés, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cytotoxicity, Immunologic, Epitope Mapping, Epitopes, HLA-A2 Antigen (immunology), Humans, Immunity, Cellular, Interferon-gamma (biosynthesis), Lymphocyte Activation, Malaria, Falciparum (immunology), Mice, Mice, Transgenic, Molecular Sequence Data, Peptides (chemistry), Peptides (immunology), Peptides (metabolism), Plasmodium falciparum (immunology), Protein Binding, Protozoan Proteins (immunology), T-Lymphocytes (immunology).
- MESH :
- chemical , biosynthesis : Interferon-gamma.
- chemical , chemistry : Peptides.
- chemical , immunology : HLA-A2 Antigen, Peptides, Protozoan Proteins.
- chemical , metabolism : Peptides.
- chemical : Epitopes.
- immunology : Malaria, Falciparum, Plasmodium falciparum, T-Lymphocytes.
- Amino Acid Sequence, Animals, Cytotoxicity, Immunologic, Epitope Mapping, Humans, Immunity, Cellular, Lymphocyte Activation, Mice, Mice, Transgenic, Molecular Sequence Data, Protein Binding.
Abstract
Localization of human MHC class I-restricted T cell epitopes in the circumsporozoite (CS) protein of the human parasite Plasmodium falciparum is an important objective in the development of antimalarial vaccines. To this purpose, we synthesized a series of overlapping synthetic 20-mer peptides, spanning the entire sequence of the 7G8 CS molecule except for the central repeat B cell domain. The P.f.CS peptides were first tested for their ability to bind to the human MHC class I HLA-A2.1 molecule on T2, a human cell line. Subsequently, the use of a series of shorter peptide analogues allowed us to determine the optimal A2.1 binding sequence present in several of the 20-mers. Binding P.f.CS peptides were further tested for their capacity to activate PBL from HLA-A2.1+ immune donors living in a malaria-endemic area. Specific IFN-gamma production was detected in the supernatant of cultures of PBL from exposed individuals. Cytotoxic T cell lines and clones were derived from the PBL of one responder, and their activity was shown to be HLA-A2.1-restricted and specific for the peptide 334-342 of the CS protein. In addition, double transgenic HLA-A2.1 x human beta 2-microglobulin mice were immunized with peptide 1-10 of the CS protein. T cells derived from immune lymph nodes displayed a peptide-specific HLA-A2.1-restricted cytolytic activity after one in vitro stimulation.
PubMed: 7535817
Affiliations:
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Le document en format XML
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Blum-Tirouvanziam</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Biochemistry, University of Lausanne, Epalinges</wicri:regionArea><wicri:noRegion>Epalinges</wicri:noRegion></affiliation></author><author><name sortKey="Servis, C" sort="Servis, C" uniqKey="Servis C" first="C" last="Servis">C. Servis</name></author><author><name sortKey="Habluetzel, A" sort="Habluetzel, A" uniqKey="Habluetzel A" first="A" last="Habluetzel">A. Habluetzel</name></author><author><name sortKey="Valmori, D" sort="Valmori, D" uniqKey="Valmori D" first="D" last="Valmori">D. Valmori</name></author><author><name sortKey="Men, Y" sort="Men, Y" uniqKey="Men Y" first="Y" last="Men">Y. Men</name></author><author><name sortKey="Esposito, F" sort="Esposito, F" uniqKey="Esposito F" first="F" last="Esposito">F. Esposito</name></author><author><name sortKey="Del Nero, L" sort="Del Nero, L" uniqKey="Del Nero L" first="L" last="Del Nero">L. Del Nero</name></author><author><name sortKey="Holmes, N" sort="Holmes, N" uniqKey="Holmes N" first="N" last="Holmes">N. Holmes</name></author><author><name sortKey="Fasel, N" sort="Fasel, N" uniqKey="Fasel N" first="N" last="Fasel">N. Fasel</name></author><author><name sortKey="Corradin, G" sort="Corradin, G" uniqKey="Corradin G" first="G" last="Corradin">G. Corradin</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cytotoxicity, Immunologic</term><term>Epitope Mapping</term><term>Epitopes</term><term>HLA-A2 Antigen (immunology)</term><term>Humans</term><term>Immunity, Cellular</term><term>Interferon-gamma (biosynthesis)</term><term>Lymphocyte Activation</term><term>Malaria, Falciparum (immunology)</term><term>Mice</term><term>Mice, Transgenic</term><term>Molecular Sequence Data</term><term>Peptides (chemistry)</term><term>Peptides (immunology)</term><term>Peptides (metabolism)</term><term>Plasmodium falciparum (immunology)</term><term>Protein Binding</term><term>Protozoan Proteins (immunology)</term><term>T-Lymphocytes (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Antigène HLA-A2 (immunologie)</term><term>Cartographie épitopique</term><term>Cytotoxicité immunologique</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Immunité cellulaire</term><term>Interféron gamma (biosynthèse)</term><term>Liaison aux protéines</term><term>Lymphocytes T (immunologie)</term><term>Paludisme à Plasmodium falciparum (immunologie)</term><term>Peptides ()</term><term>Peptides (immunologie)</term><term>Peptides (métabolisme)</term><term>Plasmodium falciparum (immunologie)</term><term>Protéines de protozoaire (immunologie)</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Épitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>HLA-A2 Antigen</term><term>Peptides</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Epitopes</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigène HLA-A2</term><term>Lymphocytes T</term><term>Paludisme à Plasmodium falciparum</term><term>Peptides</term><term>Plasmodium falciparum</term><term>Protéines de protozoaire</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Malaria, Falciparum</term><term>Plasmodium falciparum</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cytotoxicity, Immunologic</term><term>Epitope Mapping</term><term>Humans</term><term>Immunity, Cellular</term><term>Lymphocyte Activation</term><term>Mice</term><term>Mice, Transgenic</term><term>Molecular Sequence Data</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Cartographie épitopique</term><term>Cytotoxicité immunologique</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Immunité cellulaire</term><term>Liaison aux protéines</term><term>Peptides</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Localization of human MHC class I-restricted T cell epitopes in the circumsporozoite (CS) protein of the human parasite Plasmodium falciparum is an important objective in the development of antimalarial vaccines. To this purpose, we synthesized a series of overlapping synthetic 20-mer peptides, spanning the entire sequence of the 7G8 CS molecule except for the central repeat B cell domain. The P.f.CS peptides were first tested for their ability to bind to the human MHC class I HLA-A2.1 molecule on T2, a human cell line. Subsequently, the use of a series of shorter peptide analogues allowed us to determine the optimal A2.1 binding sequence present in several of the 20-mers. Binding P.f.CS peptides were further tested for their capacity to activate PBL from HLA-A2.1+ immune donors living in a malaria-endemic area. Specific IFN-gamma production was detected in the supernatant of cultures of PBL from exposed individuals. Cytotoxic T cell lines and clones were derived from the PBL of one responder, and their activity was shown to be HLA-A2.1-restricted and specific for the peptide 334-342 of the CS protein. In addition, double transgenic HLA-A2.1 x human beta 2-microglobulin mice were immunized with peptide 1-10 of the CS protein. T cells derived from immune lymph nodes displayed a peptide-specific HLA-A2.1-restricted cytolytic activity after one in vitro stimulation.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><noCountry><name sortKey="Corradin, G" sort="Corradin, G" uniqKey="Corradin G" first="G" last="Corradin">G. Corradin</name><name sortKey="Del Nero, L" sort="Del Nero, L" uniqKey="Del Nero L" first="L" last="Del Nero">L. Del Nero</name><name sortKey="Esposito, F" sort="Esposito, F" uniqKey="Esposito F" first="F" last="Esposito">F. Esposito</name><name sortKey="Fasel, N" sort="Fasel, N" uniqKey="Fasel N" first="N" last="Fasel">N. Fasel</name><name sortKey="Habluetzel, A" sort="Habluetzel, A" uniqKey="Habluetzel A" first="A" last="Habluetzel">A. Habluetzel</name><name sortKey="Holmes, N" sort="Holmes, N" uniqKey="Holmes N" first="N" last="Holmes">N. Holmes</name><name sortKey="Men, Y" sort="Men, Y" uniqKey="Men Y" first="Y" last="Men">Y. Men</name><name sortKey="Servis, C" sort="Servis, C" uniqKey="Servis C" first="C" last="Servis">C. Servis</name><name sortKey="Valmori, D" sort="Valmori, D" uniqKey="Valmori D" first="D" last="Valmori">D. Valmori</name></noCountry><country name="Suisse"><noRegion><name sortKey="Blum Tirouvanziam, U" sort="Blum Tirouvanziam, U" uniqKey="Blum Tirouvanziam U" first="U" last="Blum-Tirouvanziam">U. Blum-Tirouvanziam</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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